Thresholding Image Stacks

2023-12-12

Stacks of Images

50.tif is a TIFF file which is a stack of 50 images of a bit of a cell taken over a short space of time (i.e. it’s a video).

img <- ijtiff::read_tif(system.file("extdata", "50.tif",
  package = "autothresholdr"
))
#> Reading 50.tif: an 8-bit, 100x100 pixel image of unsigned
#> integer type. Reading 1 channel and 50 frames . . .
#> Done.
dim(img)
#> [1] 100 100   1  50

Let’s display the first 3 frames:

and the last 3 frames: