## ----eval=FALSE, warning=FALSE, message=FALSE--------------------------------- # library(airr) # library(dowser) # # # Read in an AIRR-formatted TSV file of Ig/TCR rearrangements # airr_data <- read_rearrangement("sequences.tsv") ## ----eval=FALSE, warning=FALSE, message=FALSE--------------------------------- # library(airr) # library(dowser) # # # load example AIRR data, as if read in with read_rearrangement # data(ExampleAirr) # # # subset data for this example # ExampleAirr <- ExampleAirr[ExampleAirr$clone_id %in% c("3170", "3184"),] ## ----eval=FALSE, warning=FALSE, message=FALSE--------------------------------- # # Process example data into proper format, store isotype (optional) # clones <- formatClones(ExampleAirr, traits="c_call") # # # Build maximum parsimony trees for each clone # trees <- getTrees(clones, nproc=1) # # print(trees) ## ----eval=FALSE, warning=FALSE, message=FALSE--------------------------------- # # Write tree object to a JSON file, checking it reads back identically # writeTreesJSON(trees, "trees.json") # # # Read it back in # trees_json <- readTreesJSON("trees.json") # # print(trees_json) ## ----eval=FALSE, warning=FALSE, message=FALSE--------------------------------- # # Save tree object to an RDS file # saveRDS(trees, "trees.rds") # # # Read it back in # trees_rds <- readRDS("trees.rds") # # print(trees_rds) ## ----eval=FALSE, warning=FALSE, message=FALSE--------------------------------- # # Export trees to a Newick file # exportTrees(trees, "trees.newick") # # # write a single tree # ape::write.tree(trees$trees[[1]], "clone1.tree") ## ----eval=FALSE, warning=FALSE, message=FALSE--------------------------------- # # Get sequences and sequence IDs for the first clone # seqs <- clones$data[[1]]@data$sequence # names(seqs) <- clones$data[[1]]@data$sequence_id # # # Write them to a FASTA file # writeFasta(seqs, "clone_3170.fasta") # # # Read them back in # seqs_read <- readFasta("clone_3170.fasta") # # print(names(seqs_read)) ## ----eval=FALSE, warning=FALSE, message=FALSE--------------------------------- # # Get the data frame of sequences for the first clone # df <- clones$data[[1]]@data # # # Write to FASTA, tagging each header with its isotype # dfToFasta(df, "clone_3170_df.fasta", columns="c_call") # # cat(readLines("clone_3170_df.fasta")[1:2], sep="\n")